Smai cut site

Enzymes and Inhibitors. Restriction Enzymes.

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Smai cut site

Hi all, 1 Has anyone used smaI as a RE to cut a vector for blunt end cloning? So my situation now is I'm trying to cut a plasmid bp with smaI. I over digest overnight at room temp. Hope you guys can shed some light SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. I'd definately phosphatase my plasmid in order to prevent self ligation. I recently used Antarctic Phosphatase and it workes also very good. Good luck!!. Sma I has an isoschizomer which may be a better enzyme. If you still need the blunt end, you can just do a fill-in or chew back the overhang.

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Learn more. We are excited to announce that all reaction buffers are now BSA-free. Find more details at www. Web pricing is applicable only to orders placed online at www. Notes SmaI is an isoschizomer of XmaI.

Smai cut site

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Close You've successfully logged out. Targeted Advertising Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. Top : Forum Archives : : Molecular Biology. Minimum of 8 characters Uppercase and lowercase letters At least one number. Your password reset link has expired. This product is available under our Early Access program - Learn More. Failure to heat the water will result in PEG that will take approximately 24 hours to dissolve. For Research Use Only. Add 4. SmaI is not the best RE out there, but I understand that sometimes you'd have to use it since it's your only choice. Forgot Password?

This article contains a list of the most studied restriction enzymes whose names start with S.

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