Spectra viewer

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The SpectraViewer tool enables you to visualize the spectral compatibility of fluorophores and fluorescent labelled probes. Revvity Sites Globally Select your location. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. The light source and filters for the chosen instrument will be displayed automatically. Alternatively, you can choose Custom Instrument or make no selection and add the light sources and filters manually. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu.

Spectra viewer

Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments. One of the most challenging aspects of multiplex fluorescence analysis is the selection of a combination of fluorochromes with different emission spectra. Spectral overlapping can, in fact, undermine the accuracy and validity of the experiment. It streamlines experiment design and helps to understand which fluorochromes might cause problems, saving troubleshooting time. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. Laser and filter settings can be manually added to facilitate the selection of fluorophores compatible with the instrument. Generates a final report listing selected products, all the spectral profiles of fluorophores of interest, together with lasers and filters.

To search for an appropriate filter, use the right-side menu section to search by part type, manufacturer, spectra viewer, or instrument.

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Trusted by leading Companies, Labs and Core Facilities worldwide. Spectra Viewer. FluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1, dyes from all suppliers alongside instrument-specific laser and filter configurations. Spectra Viewer and Experiment Design. A fluorophore with good separation between the excitation and emission maxima results in more reliable detection than a fluorophore with little separation. The continuous development of dyes with improved spectral profiles combined with breakthroughs in light sources, detection methods, and interference filters have paved the way to the adoption of multiplex analysis beyond the realm of flow cytometry. Acquiring large amounts of relevant biological information for each sample has become paramount also in microscopy and imaging experiments.

Spectra viewer

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Note: if an excitation source is added to the graph, the rightmost column in the information table at the bottom of the page, labeled "Peak Intensity" with the excitation source title in parentheses will show the percentage of the maximum possible intensity for the emission curve of each compound currently on the graph. Pre-loaded light sources and laser sets for microscopy and flow cytometry applications. The user may then "paste" the link into an email or other form of digital communication by either using the right mouse button or clicking the "CTRL" and "v" keys simultaneously. Then repeat the process with the bandwidth number. To use the viewer for absorbance dyes colorimetric format labels , click the left drop-down menu under "Current mode" and select "Absorbance". To search for dyes by feature, use the section on the right and enter in the desired values, then press the ENTER key. These percentages are automatically calculated for each compound currently on the graph. To remove a compound, click the "x" to the right of the compound name. To select a known filter, select one or more options on the left menu. Spectra Viewer. Cart 0 Sign In.

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Adding Filters [ Show ] To add one or more filters, click "Filter" in the "Add" submenu on the left part of the screen. You may add further fluorophores, light sources and filters at any point, or remove them by clicking X. Light sources are input in nm format and filters as - Next, select the fluorophores of interest from the Add Fluorophore drop-down menu. To search for dyes by feature, use the section on the right and enter in the desired values, then press the ENTER key. Adjusting Graph Display [ Show ] If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it. To predict the response of the fluorophores on the graph to an excitation laser, click "Predict Intensity" under the "Analyze" submenu. Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses. Name Required. How to use the SpectraViewer If you're using a Revvity imaging or detection instrument, start by selecting the instrument from the Select Machine drop-down menu. Cart 0. The display will adjust to the desired settings. A popup box will appear on the top of the page with an input box for the starting wavelength. To select all of a range of dyes, click the first desired choice, hold the SHIFT key while clicking the dye at the end of the desired range.